![]() Semisolid media: This media has 0.2-0.5% agar concentration, and due to the reduced agar concentration, it appears as a soft, jelly-like substance.Įxamples of solid media are blood agar, nutrient agar, McConkey agar, and chocolate agar. The media is not hydrolyzed by microorganisms and is free from growth-inhibiting substances. The bacterial growth on solid media varies in appearance as mucoid, round, smooth, rough, filamentous, irregular, and punctiform. Solid media are used to grow microorganisms in their full physical form, prepare bacterial pure cultures, or isolate bacteria to study colony characteristics. Sometimes, in the place of agar, some other inert solidifying agents are used, such as gellan gum. The agar-containing media solidifies at 37 ✬. Most commonly, 1.3% agar is used to prepare solid media in labs. Solid media: In these media, the agar which is an unbranched long chain of polysaccharides is added in the concentration of 1.5-2.0%.This article will focus on the composition, classification, and types of culture media used in microbiology labs to study a spectrum of microbial forms. įurthermore, it’s also essential for isolating pure cultures, storing culture stock, studying biochemical reactions, testing microbial contamination, checking antimicrobial agents and preservatives effect, testing viable count, and testing antibiotic sensitivity. ![]() Ĭulturing microorganisms is essential for diagnosing infectious diseases, obtaining antigens, developing serological assays for vaccines, genetic studies, and identification of microbial species. However, there are also microorganisms that can’t grow on a culture media at all in any condition – these are called obligate parasites. Microorganisms have varying nature, characteristics, habitat, and even nutritional requirements, thus it is impossible to culture them with one type of culture media. ![]() Balances, Scales and Weighing EquipmentĬulture media are mediums that provide essential nutrients and minerals to support the growth of microorganisms in the laboratory. ![]()
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